> Science Journals
> Cell Culture Handbook
> Cell contamination
> fluorochrome table
> stable transfection
other notes:
1000-1500 rpm for 5 mins....for freezing cells
Tissue culture media contains Calcium and Magnesium ions, foetal calf serum contains proteins that are trypsin inhibitors. Both Mg2+/Ca2+ INHIBIT TRYPSIN. The reason why we use PBS without Ca2+/Mg2+ to wash the cells prior to trypsinisation is to reduce the concentration of Divalent cations and proteins that inhibit trypsin action. EDTA is a Calcium chelator which will "mop" up the remaining divalent cations. If trypsin is allowed to stay in contact with the cells for too long a time, cell viabilty will reduce.
This should be the first principle of cell culture that you learn on day one. There are only very few cells that will detach with EDTA treatment alone.
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hum...
Friday, November 16, 2007
Friday, November 02, 2007
[NEWS] C 型肝炎
[BBC NEWS] How Hep C bypasses cell defences
"The potentially-fatal hepatitis C virus evades the body's natural defences by slipping directly from cell to cell, scientists have found. This could mean treatments aimed at interrupting its progress won't work as well as hoped. University of Birmingham researchers told a Glasgow conference that this could explain the rapid spread of the virus in some patients. "
"The potentially-fatal hepatitis C virus evades the body's natural defences by slipping directly from cell to cell, scientists have found. This could mean treatments aimed at interrupting its progress won't work as well as hoped. University of Birmingham researchers told a Glasgow conference that this could explain the rapid spread of the virus in some patients. "
Thursday, November 01, 2007
[NEWS] 殺病毒，止癌症
[SciAm] Kill the Virus, Stop the Cancer
"Researchers have for the first time shown that as many as 1.3 million cases of cancer a year may one day be successfully treated or even prevented by targeting and destroying the viruses that cause them.
Scientists at the Albert Einstein College of Medicine of Yeshiva University in New York City say the finding could pave the way for conquering human cancers that are linked to preexisting viral infections, among them liver cancer (caused by the hepatitis B and C viruses), cervical cancer (from human papillomavirus) and lymphomas caused by the Epstein–Barr virus.
They key, they say, is to find and destroy the viruses before they turn cancerous. In an attempt to do this, the researchers used a technique known as radioimmunotherapy in which radioisotopes (unstable elements that release radiation) mounted on antibodies (protein molecules akin to infection search-and-destroy missiles) are injected into the body. Once inside, researchers found that the antibodies zeroed in on their targets—viral antigens—and the radioisotopes destroyed nearby cancer cells without damaging surrounding healthy tissue."
有沒有這麼神奇阿，可以控制 radioisotope 到接近癌細胞的時候才制出放射線？如果控制不夠準確的話，還是會殺死其他好的細胞，不過這樣最壞應該也只是回到目前的治療況狀，如果能控制到只殺死癌細胞的話，算是一個加分，也許能讓病人的免疫力不會減弱吧？
"Researchers have for the first time shown that as many as 1.3 million cases of cancer a year may one day be successfully treated or even prevented by targeting and destroying the viruses that cause them.
Scientists at the Albert Einstein College of Medicine of Yeshiva University in New York City say the finding could pave the way for conquering human cancers that are linked to preexisting viral infections, among them liver cancer (caused by the hepatitis B and C viruses), cervical cancer (from human papillomavirus) and lymphomas caused by the Epstein–Barr virus.
They key, they say, is to find and destroy the viruses before they turn cancerous. In an attempt to do this, the researchers used a technique known as radioimmunotherapy in which radioisotopes (unstable elements that release radiation) mounted on antibodies (protein molecules akin to infection search-and-destroy missiles) are injected into the body. Once inside, researchers found that the antibodies zeroed in on their targets—viral antigens—and the radioisotopes destroyed nearby cancer cells without damaging surrounding healthy tissue."
有沒有這麼神奇阿，可以控制 radioisotope 到接近癌細胞的時候才制出放射線？如果控制不夠準確的話，還是會殺死其他好的細胞，不過這樣最壞應該也只是回到目前的治療況狀，如果能控制到只殺死癌細胞的話，算是一個加分，也許能讓病人的免疫力不會減弱吧？
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